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How to perform test

 HbA1c | Lansionbio

On this page

  1. Video tutorial
  2. Supplies overview
  3. Text instructions
  4. Results evaluation
  5. Disposal instructions

Video tutorial for HbA1c by Lansionbio





Supplies overview
  • 1x HbA1C Test Cassette
  • 1x 5-100µL Pipette / Capillary
  • 1x Sterile Lancet
  • 1x Alcohol Swab
  • 1x Sterile Swab
  • 1x Buffer Solution Tube
  • 1x Igloo Reader
  • 1x Lansionbio Adapter

Text instructions


STEP 1: Prepare and Disinfect the Sampling Area

Lay out all kit components. Disinfect the fingertip with the alcohol swab and let it dry.

Warm hands make blood sampling easier! Allow the test subject to warm their hands beforehand with a warm cloth, warm water, or by gently rubbing their hands together.


STEP 2: Perform a Fingerstick

Use the sterile lancet to puncture the side of the fingertip. Gently massage the finger to form a blood drop.


​

STEP 3: Remove the first droplet of blood

Wipe away the first drop of blood with a sterile swab before collecting the sample. This ensures accuracy and prevents contamination. 


STEP 4: Collect exactly 5 µL of blood

The blood will fill the pipette automatically by capillary action. The small inserted tube at the end of the pipette corresponds to the correct sample volume.

Important: Ensure the blood sample is collected properly.


​

Important

Handle the pipette gently. Use only the top part when drawing up or releasing liquid.

Hold the upper part of the pipette and avoid squeezing too hard to ensure accurate volume and prevent air bubbles.

 


STEP 5: Transfer Blood into Buffer

Gently press only the top of the pipette to release the entire blood sample into the buffer tube for proper mixing. To deliver sample from the pipette completely, gently absorb and release some buffer solution. 


STEP 8: Prepare the test cassette

Transfer the full volume from the pipette into the test cassette.


STEP 7: Collect 100 µL of the mixed blood and buffer solution

Gently press the top of the pipette to collect the mixed blood and buffer up to the indicated mark. The 100 µL correspond to the full length of the tube up to the first bubble.


STEP 6: Mix the blood with the buffer

Shake 5–10 times to ensure proper mixing and let it stand for 60 seconds.


STEP 10: Insert the test cassette halfway into the reader

Insert the test cassette halfway into the reader to enable QR code scanning. Wait until the reader completes the scan and the green indicator light turns on.

Or print the result with a mini printer 



STEP 12: Wait 5 minutes

STEP 9: Start using the device


Turn on the device using the power button located underneath.

Once the device is on, press “New Measurement” and enter the required data to begin the test.

STEP 11: Insert the test cassette fully


Once the indicator light turns green, insert the test cassette completely to start the measurement.



For the measurement to be performed, the temperature must be between 22–26 °C



Result is ready




Scan QR for quick results on your phone in PDF form

Results evaluation

Rapid HbA1c tests may differ slightly from laboratory results - this is normal and does not mean the device is faulty. Use these ranges to explain results clearly to customers and guide next steps.

Result: Error / No control line

​What it means: Test did not run correctly.

How to explain/action: Repeat the test. If the issue persists, check sample and instructions.

Result: 0

​What it means: Test did not run correctly.

How to explain/action: Repeat the test. If the issue persists, check sample and instructionsю

Result: < 5.7

​What it means: Normal glycemic control.

How to explain/action: Reassure customer. Encourage healthy habits and routine screening.

Result: 5.7 – 6.4

​What it means: Prediabetes range.

How to explain/action: Explain increased risk. Recommend lifestyle changes, follow-up testing, visit of a medical professional.


Result: ≥ 6.5

​What it means: Diabetes range.

How to explain/action: Advise medical consultation for diagnosis and management.


Key points to keep in mind:

  • Point-of-care variation can differ slightly from lab results - small differences (e.g., HbA1c just above/below a cutoff) are often within expected biological/test variability and not a device malfunction.

  • If you see 0 or error (no control line), the test may not work - repeat the test.

  • For borderline values (especially near 5.7–6.5), users should advise follow-up testing and/or clinician review rather than assume the tool is wrong.

  • HbA1c results can be affected by sample storage and environmental temperature. Studies have shown that when whole blood samples are stored at non‑optimal temperatures, measured HbA1c values can change compared to samples stored at recommended conditions like refrigeration or freezing. Improper temperature conditions during storage or prolonged exposure to heat may lead to inaccurate results. Proper handling and adherence to temperature guidelines help ensure stable and reliable HbA1c measurements.

Read more about HbA1c and about results evaluation.

Disposal instructions

  • Dispose of used cassettes, pipettes, and lancets as biohazardous waste.
  • Do not reuse any test components.
  • Reader and adapter are reusable; clean according to the official maintenance guide.

Literature

  • NIH, National Library of Medicine - Hemoglobin A1C.

  • Rohlfing CL et al. Effects of whole blood storage on hemoglobin a1c measurements with five current assay methods. Diabetes Technol Ther. 2012 Mar;14(3):271-5. doi: 10.1089/dia.2011.0136.

  • Niazpour F et al. The Effect of Blood Sample Storage Conditions on HbA1c Concentration. Clin Lab. 2019 Jul 1;65(7). doi: 10.7754/Clin.Lab.2019.190114.

Disclaimer

This information is educational and does not qualify for a medical advice. Always use local reference ranges and consult a healthcare professional for diagnosis and treatment.


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