Overslaan naar inhoud
  • +1 555-555-5556
Igloo
  • 0
  • Aanmelden
  • AR CS NL EN FI FR DE HI IT PL PT ES SV UK
  • Ons contacteren
  • Startpagina
  • Shop
  • Support

    For user


    Support requestCustomer surveyList of all test instructions QC procedureResults evaluation

    For Test Manufacturer


    Test adaptationStep 1 | Get your Igloo Step 2 | Test calibration Step 3 | Place barcode on cartridge

    For Pharmasist


    Add to Igloo Health Map Customised Report Request
  • Downloads
  • Documents
  • For businesses

     

     For Businesses


    Point-of-care businesses Pharmacies Dental clinics

    Coming soon*


    Gyms and fitness centers Telemedecine Cosmetology and beauty salons Midwifery care
  • Science hub

      

    Biomarkers


    Ferritin Vitamin D TSHLipid panelHbA1chs-CRPTestosteroneApolipoprotein BLipoprotein A

    Technology


    Igloo Reader ProDx StudioDx CareDx Cloud

    Company


    About Dx365
  • News
  • Contact
Igloo
  • 0
    • Startpagina
    • Shop
    • Support
    • Downloads
    • Documents
    • For businesses
    • Science hub
    • News
    • Contact
  • +1 555-555-5556
  • AR CS NL EN FI FR DE HI IT PL PT ES SV UK
  • Aanmelden
  • Ons contacteren

How to perform test

 HbA1c | Lansionbio

On this page

  1. Video tutorial
  2. Supplies overview
  3. Text instructions
  4. Results evaluation
  5. Disposal instructions

Video tutorial for HbA1c by Lansionbio





Supplies overview
  • 1x HbA1C Test Cassette
  • 1x 5-100µL Pipette / Capillary
  • 1x Sterile Lancet
  • 1x Alcohol Swab
  • 1x Desinfection wipe
  • 1x Buffer Solution Tube
  • 1x Igloo Reader
  • 1x Lansionbio Adapter

Included in test box:

  • 1x HbA1C Test Cassette
  • 1x 5-100µL Pipette / Capillary
  • 1x Buffer Solution Tube

Additionally needed to perform test:

  • 1x Sterile Lancet
  • 1x Desinfection wipe
  • 1x Igloo Reader
  • 1x Lansionbio Adapter

Text instructions


STEP 1: Prepare and Disinfect the Sampling Area

Lay out all kit components. Disinfect the fingertip with the alcohol swab and let it dry.

Warm hands make blood sampling easier! Allow the test subject to warm their hands beforehand with a warm cloth, warm water, or by gently rubbing their hands together.


STEP 2: Perform a Fingerstick

Use the sterile lancet to puncture the side of the fingertip. Gently massage the finger to form a blood drop.


​

STEP 3: Remove the first droplet of blood

Wipe away the first drop of blood with a sterile swab before collecting the sample. This ensures accuracy and prevents contamination. 


STEP 4: Collect exactly 5 µL of blood

The blood will fill the pipette automatically by capillary action. The small inserted tube at the end of the pipette corresponds to the correct sample volume.

Important: Ensure the blood sample is collected properly.


​

Important

Handle the pipette gently. Use only the top part when drawing up or releasing liquid.

Hold the upper part of the pipette and avoid squeezing too hard to ensure accurate volume and prevent air bubbles.

 


STEP 5: Transfer Blood into Buffer

Gently press only the top of the pipette to release the entire blood sample into the buffer tube for proper mixing. To deliver sample from the pipette completely, gently absorb and release some buffer solution. 


STEP 8: Prepare the test cassette

Transfer the full volume from the pipette into the test cassette.


STEP 7: Collect 100 µL of the mixed blood and buffer solution

Gently press the top of the pipette to collect the mixed blood and buffer up to the indicated mark. The 100 µL correspond to the full length of the tube up to the first bubble.


STEP 6: Mix the blood with the buffer

Shake 5–10 times to ensure proper mixing and let it stand for 60 seconds.


STEP 10: Insert the test cassette halfway into the reader

Insert the test cassette halfway into the reader to enable QR code scanning. Wait until the reader completes the scan and the green indicator light turns on.

Or print the result with a mini printer 



STEP 12: Wait 5 minutes

STEP 9: Start using the device


Turn on the device using the power button located underneath.

Once the device is on, press “New Measurement” and enter the required data to begin the test.

STEP 11: Insert the test cassette fully


Once the indicator light turns green, insert the test cassette completely to start the measurement.



For the measurement to be performed, the temperature must be between 22–26 °C



Result is ready




Scan QR for quick results on your phone in PDF form

Results evaluation

Rapid HbA1c tests may differ slightly from laboratory results - this is normal and does not mean the device is faulty. Use these ranges to explain results clearly to customers and guide next steps.

Result: Error / No control line

​What it means: Test did not run correctly.

How to explain/action: Repeat the test. If the issue persists, check sample and instructions.

Result: 0

​What it means: Test did not run correctly.

How to explain/action: Repeat the test. If the issue persists, check sample and instructions.

Result: < 5.7

​What it means: Normal glycemic control.

How to explain/action: Reassure customer. Encourage healthy habits and routine screening.

Result: 5.7 – 6.4

​What it means: Prediabetes range.

How to explain/action: Explain increased risk. Recommend lifestyle changes, follow-up testing, visit of a medical professional.


Result: ≥ 6.5

​What it means: Diabetes range.

How to explain/action: Advise medical consultation for diagnosis and management.

Blood Glucose & HbA1c: Results look different


Blood glucose and HbA1c measure different aspects of glycaemia:

  • Blood glucose reflects the glucose level at a specific moment
  • HbA1c reflects the average glucose exposure over the previous ~8-12 weeks

Because they measure different timeframes and are influenced by different biological factors, results may sometimes not match. This is common and does not automatically indicate an error or disease change.


Scenario 1: High blood glucose but low HbA1c


Possible explanation:
This pattern may occur when recent glucose levels have increased but the longer-term average is still lower, or when HbA1c is affected by factors that reduce its value.

Examples include:
  • recent change in diet, illness, stress, or medication
  • short-term hyperglycaemia not yet reflected in HbA1c
  • conditions shortening red blood cell lifespan (e.g. some anaemias)
  • chronic kidney disease or certain medications affecting HbA1c reliability

What pharmacists can advise customers:
  • repeat glucose monitoring as recommended
  • review recent lifestyle or medication changes
  • discuss results with their general practicioner or diabetes team
  • consider follow-up testing if mismatch persists


Scenario 2: Low blood glucose but high HbA1c


Possible explanation:
This pattern may occur when current glucose is improved but previous levels were higher, or when HbA1c is influenced by factors that increase its value independently of glucose.

Examples include:
  • recent improvement in glucose control
  • iron deficiency anaemia
  • haemoglobin variants (genetic differences in the structure of haemoglobin)
  • longer red blood cell lifespan
  • biological differences between individuals in haemoglobin glycation

What pharmacists can advise customers:
  • continue regular monitoring
  • avoid interpreting a single reading alone
  • discuss possible causes with their healthcare professional
  • follow recommended repeat laboratory testing if advised

Key points to keep in mind:

  • Point-of-care variation can differ slightly from lab results - small differences (e.g., HbA1c just above/below a cutoff) are often within expected biological/test variability and not a device malfunction.

  • If you see 0 or error (no control line), the test may not work - repeat the test.

  • For borderline values (especially near 5.7–6.5), users should advise follow-up testing and/or clinician review rather than assume the tool is wrong.

  • HbA1c results can be affected by sample storage and environmental temperature. Studies have shown that when whole blood samples are stored at non‑optimal temperatures, measured HbA1c values can change compared to samples stored at recommended conditions like refrigeration or freezing. Improper temperature conditions during storage or prolonged exposure to heat may lead to inaccurate results. Proper handling and adherence to temperature guidelines help ensure stable and reliable HbA1c measurements.

Read more about HbA1c and about results evaluation.

Disposal instructions

  • Dispose of used cassettes, pipettes, and lancets as biohazardous waste.
  • Do not reuse any test components.
  • Reader and adapter are reusable; clean according to the official maintenance guide.

Literature

  • NIH, National Library of Medicine - Hemoglobin A1C.

  • Rohlfing CL et al. Effects of whole blood storage on hemoglobin a1c measurements with five current assay methods. Diabetes Technol Ther. 2012 Mar;14(3):271-5. doi: 10.1089/dia.2011.0136.

  • Niazpour F et al. The Effect of Blood Sample Storage Conditions on HbA1c Concentration. Clin Lab. 2019 Jul 1;65(7). doi: 10.7754/Clin.Lab.2019.190114.

  • Mann, DM, et al. Understanding discordance between HbA1c and blood glucose measurements. doi: 10.2337/dc12-1479

Disclaimer

This information is educational and does not qualify for a medical advice. Always use local reference ranges and consult a healthcare professional for diagnosis and treatment.


Legal

  • Imprint
  • Legal info
  • Privacy policy
  • Cookie policy
  • Sales terms and conditions


Klantenservice

  • Support Request Center
  • Contact
  • Downloads
  • Documents
  • Test Instructions
  • Test Manufacturer Instructions
Phone support

  • +49 40 3680 74990
Social Networks

Social Media

© Dx365 GmbH - Algemene voorwaarden - Privacybeleid
الْعَرَبيّة | Čeština | Nederlands | English (US) | Suomi | Français | Deutsch | हिंदी | Italiano | Język polski | Português | Español | Svenska | українська
Aangeboden door Odoo - De #1 Open source e-commerce

Respecting your privacy is our priority.

Allow the use of cookies from this website on this browser?

We use cookies to provide improved experience on this website. You can learn more about our cookies and how we use them in our Cookiebeleid.

Allow all cookies
Only allow essential cookies