How to perform test

CRP | Lansionbio

Video tutorial for hs-CRP/CRP by Lansionbio

Supplies overview

1x CRP Test Cassette
1x 5-100µL Pipette / Capillary
1x Sterile Lancet
1x Alcohol Swab
1x Sterile Swab
1x Buffer Solution Tube
1x Igloo Reader
1x Lansionbio Adapter

Text instructions

STEP 1: Prepare and Disinfect the Sampling Area

Lay out all kit components. Disinfect the fingertip with the alcohol swab and let it dry.

Warm hands make blood sampling easier! Allow the test subject to warm their hands beforehand with a warm cloth, warm water, or by gently rubbing their hands together.

STEP 2: Perform a Fingerstick

Use the sterile lancet to puncture the side of the fingertip. Gently massage the finger to form a blood drop.

STEP 3: Remove the first droplet of blood

Wipe away the first drop of blood with a sterile swab before collecting the sample. This ensures accuracy and prevents contamination.

STEP 4: Collect exactly 5 µL of blood

The blood will fill the pipette automatically by capillary action. The small inserted tube at the end of the pipette corresponds to the correct sample volume.

Important: Ensure the blood sample is collected properly.

Important

Handle the pipette gently. Use only the top part when drawing up or releasing liquid.

Hold the upper part of the pipette and avoid squeezing too hard to ensure accurate volume and prevent air bubbles.

STEP 5: Transfer Blood into Buffer

Gently press only the top of the pipette to release the entire blood sample into the buffer tube for proper mixing. To deliver sample from the pipette completely, gently absorb and release some buffer solution.

STEP 6: Mix the blood with the buffer

Shake 5–10 times to ensure proper mixing.

Or print the result with a mini printer

Result is ready

STEP 7: Collect 100 µL of the mixed blood and buffer solution

Gently press the top of the pipette to collect the mixed blood and buffer up to the indicated mark.

Transfer the full volume from the pipette into the test cassette.

STEP 9: Start using the device

Turn on the device using the power button located underneath.

Once the device is on, press “New Measurement” and enter the required data to begin the test.

STEP 10: Insert the test cassette halfway into the reader

Insert the test cassette halfway into the reader to enable QR code scanning. Wait until the reader completes the scan and the green indicator light turns on.

Once the indicator light turns green, insert the test cassette completely to start the measurement.

STEP 12: Wait 3 minutes

Scan QR for quick results on your phone in PDF form

Results evaluation

Rapid hs-CRP/CRP tests may differ slightly from laboratory results - this is normal and does not indicate a faulty device. Use the following ranges to explain results clearly to customers and guide appropriate next steps. Reference ranges may vary slightly by lab and test method.

Result: Error / No control line

What it means: Test did not run correctly.

How to explain/action: Repeat the test. If the issue persists, check sample and instructions.

Result: 0 - 1 mg/L

What it means: Low risk, low inflammation - indicates low risk for cardiovascular disease.

How to explain/action: Reassure customer, suggest to maintain healthy lifestyle and routine health monitoring.

Result: 1 – 3 mg/L

What it means: Average risk, moderate inflammation - average risk for cardiovascular disease.

How to explain/action: Recommend healthy lifestyle changes and follow-up testing as well as consultation with a healthcare provider.

Result: > 3 mg/L

What it means: High risk, elevated inflammation - higher risk for cardiovascular disease or active inflammation/infection.

How to explain/action: Advise customer to consult healthcare provider for further evaluation and possible additional testing.

Key points to keep in mind:

Point-of-care results can differ slightly from lab tests. Small variations are common and do not mean the device is faulty.
If you see an error or no control line, the test might not have worked - always repeat.
For borderline or unusual values, advise customers to seek follow-up testing and medical advice instead of assuming device problems.
Ferritin levels can rise more slowly and stay elevated longer during inflammation compared to CRP. While CRP peaks early and drops quickly as inflammation resolves, ferritin may remain high even after CRP returns to normal. This is normal and should be considered when interpreting ferritin results.

Read more about hs-CRP, ferritin, and about results evaluation.

Disposal instructions

Dispose of used cassettes, pipettes, and lancets as biohazardous waste.
Do not reuse any test components.
Reader and adapter are reusable; clean according to the official maintenance guide.

Literature

Mayo clinic - C-reactive protein test
Medline Plus - C-reactive protein
Testing - hs-CRP Test (C-Reactive Protein High-Sensitivity)
Agency for Healthcare Research and Quality (AHRQ) - Assessing Cardiovascular Risk: Guideline Synthesis
Beyhoff N et al. Prognostic Impact of High-Sensitivity C-Reactive Protein in Patients Undergoing Percutaneous Coronary Intervention According to BMI. J Am Coll Cardiol Intv. 2020 Dec, 13 (24) 2882–2892.https://doi.org/10.1016/j.jcin.2020.09.024

Disclaimer

This information is educational and does not qualify for a medical advice. Always use local reference ranges and consult a healthcare professional for diagnosis and treatment.