How to perform test

Ferritin | Lansionbio

Video tutorial for Ferritin by Lansionbio

Supplies overview

1x Ferritin Test Cassette
1x Fixed-Volume Capillary Pipette
1x Sterile Lancet
1x Alcohol Swab
1x Sterile Swab
1x Buffer Solution Tube
1x Igloo Reader
1x Lansionbio Adapter

Text instructions

STEP 1: Prepare and Disinfect the Sampling Area

Lay out all kit components. Disinfect the fingertip with the alcohol swab and let it dry.

Warm hands make blood sampling easier! Allow the test subject to warm their hands beforehand with a warm cloth, warm water, or by gently rubbing their hands together.

STEP 2: Perform a Fingerstick

Use the sterile lancet to puncture the side of the fingertip. Gently massage the finger to form a blood drop.

STEP 3: Remove the first droplet of blood

Wipe away the first drop of blood with a sterile swab before collecting the sample. This ensures accuracy and prevents contamination.

STEP 4: Collect exactly 5 µL of blood

The blood will fill the pipette automatically by capillary action. The small inserted tube at the end of the pipette corresponds to the correct sample volume.

Important: Ensure the blood sample is collected properly.

Important

Handle the pipette gently. Use only the top part when drawing up or releasing liquid.

Hold the upper part of the pipette and avoid squeezing too hard to ensure accurate volume and prevent air bubbles.

STEP 5: Transfer Blood into Buffer

Gently press only the top of the pipette to release the entire blood sample into the buffer tube for proper mixing. To deliver sample from the pipette completely, gently absorb and release some buffer solution.

STEP 6: Mix the blood with the buffer

Shake 5–10 times to ensure proper mixing.

STEP 7: Collect the mixed blood and buffer solution

Gently press the top of the pipette to collect the mixed blood and buffer up to the indicated mark.

STEP 8: Prepare the test cassette

Transfer the full volume from the pipette into the test cassette.

STEP 9: Start using the device

Turn on the device using the power button located underneath.

Once the device is on, press “New Measurement” and enter the required data to begin the test.

STEP 10: Insert the test cassette halfway into the reader

Insert the test cassette halfway into the reader to enable QR code scanning. Wait until the reader completes the scan and the green indicator light turns on.

STEP 11: Insert the test cassette fully

Once the indicator light turns green, insert the test cassette completely to start the measurement.

STEP 12: Wait 15 minutes

Result is ready

Scan QR for quick results on your phone in PDF form

Or print the result with a mini printer

Results evaluation

Rapid ferritin tests may differ slightly from laboratory results - this is normal and does not mean the device is faulty. Use these ranges to explain results clearly to customers and guide next steps.

Result: Error / No control line

What it means: Test did not run correctly.

How to explain/action: Repeat the test. If the issue persists, check sample and instructions.

Result: 0

What it means: Test did not detect ferritin - ferritin cincentration is either below detection limit or the test is invalid.

How to explain/action: Repeat the test. If the result is still 0, this suggests very low ferritin; explain to the customer possible iron deficiency. Recommend seeing a healthcare professional for diagnosis and supplementation.

Result: < 30 ng/mL

What it means: Low ferritin- possible iron deficiency.

How to explain/action: Inform customer of possible iron deficiency. Recommend seeing a healthcare professional for diagnosis and supplementation.

Result: 30 - 300 ng/mL

What it means: Normal ferritin level.

How to explain/action: Reassure customer that ferritin is within normal range. Encourage routine health monitoring.

Result: > 300 ng/mL

What it means: High ferritin - may indicate inflammation or iron overload.

How to explain/action: Advise customer to consult a healthcare professional to investigate causes.

Key points to keep in mind:

Point-of-care results can differ slightly from lab tests. Small variations (e.g., ferritin 50 vs 70) are common and do not mean the device is faulty.
If you see 0 or error (no control line), the test might not work - always repeat.
For borderline or unusual values, advise customers to seek follow-up testing and medical advice instead of assuming device problems.
Ferritin levels can rise more slowly and stay elevated longer during inflammation compared to CRP. While CRP peaks early and drops quickly as inflammation resolves, ferritin may remain high even after CRP returns to normal. This is normal and should be considered when interpreting ferritin results.

Read more about Ferritin and about results evaluation.

Disposal instructions

Dispose of used cassettes, pipettes, and lancets as biohazardous waste.
Do not reuse any test components.
Reader and adapter are reusable; clean according to the official maintenance guide.

Literature

NIH Office of Dietary Supplements - Iron: Health Professional Fact Sheet.
NIH Office of Dietary Supplements - Iron: Consumer Fact Sheet.
Mayo Clinic – Ferritin test.

Disclaimer

This information is educational and does not qualify for a medical advice. Always use local reference ranges and consult a healthcare professional for diagnosis and treatment.